Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 22;5:10510. doi: 10.1038/srep10510

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Polymerase activities of R-DK/49 or R-GS/65 with different PA mutations in a minigenome assay. Polymerase complex and NP expression plasmids together with the minigenome firefly luciferase reporter plasmid phumPolI-T-Luc or paviPolI-T-Luc were transfected into 293T cells (A) or DF1 (B) cells, respectively. Data are expressed as the mean ± standard deviation (SD) for three independent experiments and are standardized to the activity of R-DK/49 (100%). Statistical significance was determined using the unpaired Student’s t-test with two-tailed analysis (GraphPad Software, La Jolla, CA). *p < 0.01, **p < 0.001, ***p < 0.0001. Negative control, transfection of firefly luciferase reporter only.