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. 2015 May 22;5:10121. doi: 10.1038/srep10121

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(a and b) Western blotting of BDNF expression in colon of mice (***P < 0.001). (c) Immunohistochemical staining of BDNF in epithelium (Black arrowheads) and mucosa in colon of mice (Bars: 50 μm). (d) Measurement of visceral sensitivity to colorectal distension in mice by using electromyography recording (^*P < 0.05, ^***P < 0.001 IBS-D FSN vs. control FSN; ^†††P < 0.001, IBS-D FSN + FUT-175 or ENMD-1068 or TrkB/Fc vs. IBS-D FSN; n = 8 per group; AUC: area under curve). The gels were run under the same experimental conditions. Cropped gels/blots are presented (full-length gels/blots are shown in suppl. Fig. S1 with indicated cropping lines).