Table 1.
Sources and use of recombinant proteins, antibodies and reagents.
| Materials | Manufacturer | Concentration use |
|---|---|---|
|
| ||
| Recombinant human (rh) protein heterodimer −2/−7 | R&D System Inc. (Minneapolis, MN, USA). | 100 ng/mL |
| Rh Noggin | R&D System Inc. (Minneapolis, MN, USA). | 500 ng/mL |
| Rh TGF-β3 | R&D System Inc. (Minneapolis, MN, USA). | 10 ng/mL |
| Monoclonal anti-osteopontin antibody (ab) | R&D System Inc. (Minneapolis, MN, USA). | 1/500 (v/v) |
| Monoclonal anti-peroxysome proliferators-activated γ ab | R&D System Inc. (Minneapolis, MN, USA). | 1/500 (v/v) |
| Enamel Matrix Derivative (EMD) | Straumann (Basel, Switzerland). Developmental Hybridoma Study Bank (Iowa City, IO, USA). |
50 μg/mL |
| Monoclonal anti-collagen II unconjugated ab | Developmental Hybridoma Study Bank (Iowa City, IO, USA). | 1/100 (v/v) |
| Monoclonal anti CD106 unconjugated ab | 1/100 (v/v) | |
| 7-amino actinomycin D | Ebiosciences (Paris, France). | 1/10 (v/v) |
| Monoclonal anti CD 14, 34, 45, 73, 79a, 90, 105, HLA-DR, isotype matched negative control abs | Ebiosciences (Paris, France). | 1/10 (v/v) |
| Monoclonal anti CD146 and anti MSCA-1 conjugated abs | Miltenyi Biotec (Paris, France). | 1/10 (v/v) |
| Monoclonal anti MSCA-1 unconjugated ab | Miltenyi Biotec (Paris, France). | 1/100 (v/v) |
| Rabbit anti CD146 unconjugated ab | Anaspec (Fremont CA, USA). | 1/100 (v/v) |
| Rabbit and mouse IgG unconjugated ab | Serotec (Cergy Saint-Christophe, France). | 1/100 (v/v) |
| Monoclonal anti CD106 conjugated ab | BD-Biosciences (Bedford, MA) | 1/10 (v/v) |
| ITS-Premix | BD-Biosciences (Bedford, MA) | 50 mg/mL |
| ELF97 endogenous phosphatase detection kit | Invitrogen (Carlsbad, CA, USA). | 1/50 (v/v) |
| Recombinant poly(His) tagged mouse 180 amino acid amelogenin (rp(H)M180) | Home-made material | 5 μg/mL |
| Monoclonal anti STRO-4 unconjugated ab | Home-made material | 1/10 (v/v) |
| Penicillin, streptomycin, phosphate buffer saline, α and D-MEM, fetal calf serum, Hoechst 33342 | Invitrogen (Carlsbad, CA, USA). | |