Figure 1. IL-17⁺, IFN-γ⁺, and IL-17⁺IFN-γ⁺ CD4⁺ cells are found in the lamina propria of colitic mice.

Naïve splenic CD4⁺ T cells from CBir1 Tg mice were i.v. injected into Rag^−/− mice. Four weeks post T-cell transfer, the severity of colonic inflammation was assessed by histological analysis after hemytoxlin and eosin staining. (A) Histopathology of colon (original magnification, 20×). (B) Pathological scores of accessed colons. Bars show mean +/− SEM of 4 mice from a single experiment representative of 4 experiments. (C) Flow cytometry of CD4⁺ cells isolated from colon lamina propria of Rag^−/− recipient measured for expression of IFN-γ and IL-17. One representative plot out of 4 experiments is shown.