Fig 5.

Sedimentation of the modification mutants.A. Mutations in the modification genes led cells to sediment in liquid culture with the exception of CD0243, this phenotype was restored by complementation in trans.B and C. This was quantified during growth by measuring the OD₆₀₀ of the top 1 ml of culture (top – continuous lines) and a well mixed culture (mix – broken lines), demonstrating sedimentation in the CD0240, CD0241, CD0242 and CD0244 mutants, confirming the observations in A.D. The sedimentation in double mutants of CD0241 and fliC, CD0242 and fliC and CD0244 and fliC were also quantified. These double mutants had a growth profile similar to the fliC clostron mutant (B).