Table 3.
Summary of glycan modifications of C. difficile 630 flagellin glycosylation mutants
| Strain/plasmid | Detected glycopeptides (precursor m/z) | Glycan masses and predicted composition (Da) |
|---|---|---|
| 630Δerm | Various peptides | 398 |
| CD0241 mutant | LLDGTSSTIR (633.32+) | 203 |
| TMVSSLDAALK (669.82+) | 203 | |
| CD0241 mutant p0241_comp | AGGTTGTDAAK (577.32+) | 203 |
| TMVSLDAALK (697.42+) | 203 | |
| TMVSLDAALK (775.32+) | 398 (203-80-115) | |
| LLDGTSSTIR (633.32+) | 203 | |
| LLDGTSSTIR (730.82+) | 398(203-80-115) | |
| CD0242 mutant | TMVSSLDAALK+ox (677.82+) | 203 |
| CD0242 mutant p0242_comp | TMVSSLDAALK (679.32+) | 203 |
| 384(203-80-101) | ||
| 398(203-80-115) | ||
| CD0243 mutant | LLDGTSSTIR (633.32+) | 203 |
| LLDGTSSTIR (732.82+) | 384(203-80-101) | |
| TMVSSLDAALK (679.32+) | 203 | |
| TMVSSLDAALK (724.22+) | 384(203-80-101) | |
| CD0244 mutant | TMVSSLDAALK (679.32+) | 203 |
| TMVSSLDAALK (775.52+) | 398(203-80-115) | |
| LLDGTSSTIR (730.82+) | 398(203-80-115) | |
| CD0244 mutant p0244_comp | LLDGTSSTIR (730.82+) | 398(203-80-115) |
| TMVSSLDAALK (775.52+) | 398(203-80-115) |