Table 1.
Parasite | Exflagellating centers/100 male gametocytes | Male gametocyte nuclear size (au) | Male gametocytes with tubulin protrusions | Released microgametes/100 male gametocytes | Flagella containing DNA |
---|---|---|---|---|---|
wt | 56 ± 13 | 150 ± 21 | 48 ± 9% | 61 ± 10 | 92 ± 5% |
Δsas6 | 0* | 149 ± 24 | 28 ± 7%* | 2 ± 0.6* | 3 ± 0.9%* |
wt-gfp | 68 ± 23 | 145 ± 18 | 56 ± 12% | 69 ± 18 | 93 ± 11% |
Δsas6-gfp | 0* | 153 ± 17 | 30 ± 14%* | 4 ± 1* | 5 ± 0.5%* |
Mean results of 3 different biological replicates. Motile exflagellating centre analysis was performed in slides using bright-field microscopy at 20 mpa. Nuclear size examination was performed using ImageJ on DAPI stained images of gametocytes fixed at 10 mpa as in Fig. 3. Flagellar protrusion, flagellar release and nuclear size analysis were performed with fluorescent microscopy in gametocytes fixed at 15 mpa as in Fig. 3. au – ImageJ arbitrary units.
Asterisk indicates statistically significant differences in Student's t-test with P-values lower than 0.05.