Table 3.

Analysis of transmission of Δsas6 by direct feed

Parasite	Mouse identity #/# fed mosquitoes	Mouse parasitaemia
wt	1/31	Positive
	2/29	Positive
	3/25	Positive
Δsas6	1/36	Negative
	2/40	Negative
	3/37	Negative
wt-gfp	1/10	Positive
	2/12	Positive
	3/17	Positive
Δsas6-gfp	1/25	Negative
	2/37	Negative
	3/32	Negative
	4/30	Negative

For each replicate, mice with similar parasitaemias were fed to An. stephensi mosquitoes. Fully fed mosquitoes were kept for 21 days and allowed to bite on naïve TO mice. Infection of naïve mice was examined by Giemsa staining of the blood after the bite.