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FK-3000 effectively blocks NF-κB translocation from cytoplasm to nucleus. (A) In untreated MDA-MB-231 cells NF-κB p65 proteins [green color (b)] are localized primarily in the nucleus [blue color (a)]. The merged image (c) confirms the NF-κB p65 protein localization. (B) In the 5.0 μg/ml FK-3000-treated cells, NF-κB p65 proteins (b) were almost exclusively localized to the cytoplasm (a). The merged image (c) confirms that the NF-κB p65 proteins are not in the cell nucleus.
