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Substrate specificities of triphosphate form of dThd, FTD, dUrd and FdUrd for DUT.
| Substrate | Production of 5′-monophosphate (pmol/min/100 ng protein) |
|---|---|
| dTTP | N.D. |
| F3dTTP | N.D. |
| dUTP | 41.4 |
| FdUTP | 34.8 |
Thirty micromolar triphosphates were incubated for 30 min at 37°C in buffer containing 100 ng recombinant dUTPase. Samples were analyzed by HPLC to quantify triphosphate and monophosphate formation. N.D., not detectable.
