Figure 4.
Nobiletin induces a concentration-dependent decrease of VEGF production from human ovarian cancer cells. The human ovarian cancer cell line OVCAR-3 (A), and A2780/CP70 (B) were treated with varying concentrations of nobiletin for 16 h. Subsequently, the VEGF levels in the supernatant was measured by ELISA. A total of three independent experiments, each in triplicates, were assayed, and the mean VEGF protein level from each duplicate was used for statistical analysis. *P<0.05 as compared to control. **P<0.01 as compared to control.