Figure 3.

Dysregulation of CADM1 affects cell proliferation in cervical cancer cells. For cell proliferation analysis, 0.5×10⁴ cells/well were incubated with various concentrations of 5-aza-dC (A) or transfected with pcDNA3.1-CADM1 (CADM1) or empty vector (Mock) (B) in a 96-well plate for 68 h, and incubated further with the CCK-8 reagent for 4 h. (C) Western blotting was used to detect the overexpressed CADM1 protein after 5-aza-dC treatment (from 1 to 100 μM). Actin and p53 were used as controls of protein loading and of 5-aza-dC effect, respectively.