Figure 5.

The phosphorylation of p53 by 5-FU upregulated CADM1. The time-dependent effect of 5-FU on the levels of the CADM1, phospho-p53, p53, and β-actin proteins in the C33A and SiHa cervical carcinoma cell lines was determined by western blot analysis. Equal amounts of the cell lysate from each cell line treated with 5-FU (50 μM) at the indicated time were separated on a 10% Tris-glycine gel and immunoblot analysis was performed using specific antibodies, including anti-CADM1, anti-phospho-p53, anti-p53, and anti-β-actin antibodies, respectively.