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. Author manuscript; available in PMC: 2015 Oct 14.
Published in final edited form as: Nat Commun. 2015 Apr 14;6:6656. doi: 10.1038/ncomms7656

Figure 7. HKL blocks differentiation of wild-type cardiac fibroblast to myofibroblasts, but not SIRT3-KO fibroblasts.

Figure 7

(A, B) Primary cultures of mouse cardiac fibroblasts obtained from wild-type (WT) and SIRT3KO mice were treated with 100 nM Ang in the presence or absence of HKL for 72 hrs. Cells were immunostained for α-SMA and collagen-1; Scale 10μm. (C) Cell lysates were prepared from another set of plates and subjected to immunostaining for α-SMA. For loading control the blot was probed with an anti-GAPDH antibody.