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. Author manuscript; available in PMC: 2015 May 22.
Published in final edited form as: Expert Rev Mol Diagn. 2009 Apr;9(3):243–257. doi: 10.1586/erm.09.10

Table 2.

List of DNA methylation assays tested in bodily fluids samples

Method Specimen Gene/Gene Panel Sensitivity Specificity Ref.
MSP Urine GSTP1 73 98 (93)
MSP Urine GSTP1 79 NR (94)
QMSP Urine GSTP1,RASSF1a, RAR-β2, APC 86 89 (95)
QMSP Urine GSTP1 75 98 (96)
QMSP Urine p16, ARF, MGMT, GSTP1 87 100 (12)
Multiplexed
QMSP
Urine GSTP1, RARB, APC 55 80 (97)
MSP Serum RASSF1, RARB2, GSTP1 28 100 (98)
MSP Serum GSTP1
AR
14-3-3sigma
32
40
87
100
73
45
(99)
QMSP Serum MDR1
EDNRB
RAR-β2
GTSP1
NEP
RASSF1A
83
50
39
28
17
17
100
100
100
100
100
100
(100)
MSRE
QMSP
Serum GSTP1, TIG1, PTGS2, Reprimo 42-47 92 (101)

MSP: Methylation specific PCR. It is gel-based, not highthroughput and subjective

QMSP: Quantitative-methylation specific PCR, automated, high-throughput and presumably more specific Mutiplexed PCR: High-throughput, cost-effective, can’t done more than 4 genes due to limitation in dye chemistry in real-time PCR. MSRE: methylation-sensitive restriction endonucleases; NR=not reported