Table 2.
Method | Specimen | Gene/Gene Panel | Sensitivity | Specificity | Ref. |
---|---|---|---|---|---|
MSP | Urine | GSTP1 | 73 | 98 | (93) |
MSP | Urine | GSTP1 | 79 | NR | (94) |
QMSP | Urine | GSTP1,RASSF1a, RAR-β2, APC | 86 | 89 | (95) |
QMSP | Urine | GSTP1 | 75 | 98 | (96) |
QMSP | Urine | p16, ARF, MGMT, GSTP1 | 87 | 100 | (12) |
Multiplexed QMSP |
Urine | GSTP1, RARB, APC | 55 | 80 | (97) |
MSP | Serum | RASSF1, RARB2, GSTP1 | 28 | 100 | (98) |
MSP | Serum | GSTP1 AR 14-3-3sigma |
32 40 87 |
100 73 45 |
(99) |
QMSP | Serum |
MDR1 EDNRB RAR-β2 GTSP1 NEP RASSF1A |
83 50 39 28 17 17 |
100 100 100 100 100 100 |
(100) |
MSRE QMSP |
Serum | GSTP1, TIG1, PTGS2, Reprimo | 42-47 | 92 | (101) |
MSP: Methylation specific PCR. It is gel-based, not highthroughput and subjective
QMSP: Quantitative-methylation specific PCR, automated, high-throughput and presumably more specific Mutiplexed PCR: High-throughput, cost-effective, can’t done more than 4 genes due to limitation in dye chemistry in real-time PCR. MSRE: methylation-sensitive restriction endonucleases; NR=not reported