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. 2015 May 22;10(5):e0126149. doi: 10.1371/journal.pone.0126149

Fig 4. PTEN decreased Snail expression through inactivation of PI3K/Akt/GSK-3β signaling.

Fig 4

(A) Expression of Snail and Slug detected by western blot analysis in KYSE-150 cells transfected with siPTEN or vehicle, or KYSE-150/RR cells transfected with pcDNA-PTEN or pcDNA3.0. (B) Expression of Snail were detected by qRT-PCR, data shown as mean±SD. (C) Representative western blot analysis showed expression of Akt, p-Akt, GSK-3β and p-GSK-3β. Data shown represent three different experiments. siPTEN is short for siRNA-PTEN. (D) Representative western blot analysis showed expression of Akt, p-Akt, GSK-3β, p-GSK-3β, Snail and E-cadherin in KYSE-150/RR cells with or without the phosphatidylinositol 3 kinase (PI3K) inhibitor, LY294002 (40 μM).