Fig. 3.

Lack of ESAM increases platelet aggregation. Platelet aggregation was performed using platelets from wild-type or ESAM^−/− mice. (A–C) Representative aggregation tracings for the indicated concentrations of (A) collagen, (B) ADP and (C) the PAR-4 agonist peptide AYPGQV. Arrows indicate addition of agonist. Tracings are representative of at least three independent experiments performed for each condition. (D) Quantitative analysis of aggregation in response to AYPGQV. Data are reported as the extent of aggregation at each agonist concentration normalized to the maximum response in each experiment (max = 100%). Values are mean ± SEM and the numbers at the base of the columns indicate the number of mice in each group.