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. Author manuscript; available in PMC: 2015 May 22.
Published in final edited form as: Mol Carcinog. 2005 May;43(1):18–30. doi: 10.1002/mc.20091

Figure 2.

Figure 2

Expression of Cux-1, p21, and p27 protein in livers from wild type and transgenic mice. Fifty micrograms of nuclear extract prepared from 8-month-old transgenic and wild type livers was subjected to SDS–PAGE and transferred to nitrocellulose membranes. The presence of Cux-1, p21, p27, and β-actin (as a loading control) was detected by Western blot analysis as described in the ‘‘Materials and Methods.’’ Cux-1 protein was elevated in the transgenic livers compared to wild type. p21 expression was decreased in the transgenic livers, compared to wild type, while p27 expression was unchanged.