Table 3. Significance of hydrophobic temperature dependence pooled.

amino acid class	p-value contacts	p-value surface	ΔΔG contacts	ΔΔG surface
hydrophobic	< 0.01	< 0.01	0.10	0.32
polar	< 0.01	0.23	-0.05	0.13
charged	< 0.01	0.80	-0.06	-0.04
aromatic	0.04	< 0.01	0.06	0.32
other	0.32	< 0.01	0.02	0.41

The difference in free energy estimates (ΔΔG) between the lowest temperature bin (265–290K) and room temperature (297–299K) is shown together with its significance (p-value) for each class of amino acids. The significance was tested using a resampling procedure. The amino acids are pooled according to defined classes; the free energy estimates are not reference corrected.