Table 2. Analysis of the different samples collected from VACV-GP2–infected mice.
Feces, blood, oral swabs (Oss), and tissues were collected at different times, and the nested-PCR, IHC, and PRNT techniques were performed.
| Time (d.p.i) | Feces (PCR) | OS (PCR) | Blood (PCR) | Tissues (PCR) | IHC | PRNT |
|---|---|---|---|---|---|---|
| T1 | - | - | - | - | - | - |
| T2 | N | P | N | Lu, To, To | Lu | N |
| T3 | - | - | - | - | - | - |
| T4 | N | P | - | - | - | - |
| T5 | - | - | P | Ki, He | Lu, Ki, Sp, Il | N |
| T6 | P | P | - | - | - | - |
| T7 | - | - | - | - | ||
| T8 | P | P | - | - | - | - |
| T9 | - | - | - | - | ||
| T10 | N | P | P | Ki, Lu, Li, He, To | Lu, Ki, Sp, Il, Li, ML | N |
| T11 | - | - | - | - | - | - |
| T12 | N | N | N | - | - | - |
| T13 | - | - | - | - | - | - |
| T14 | N | N | N | - | - | - |
| T15 | - | - | - | |||
| T16 | N | N | N | - | - | - |
| T17 | - | - | - | |||
| T18 | N | N | N | - | - | - |
| T19 | - | - | - | - | - | - |
| T20 | N | N | P | Sp | Lu, Ki, Sp, Il, ML, Li, Bl | NA |
| T21 | - | - | - | - | - | - |
| T22 | N | N | N | - | - | - |
| T23 | - | - | - | - | - | - |
| T24 | N | N | N | - | - | - |
| T25 | - | - | - | - | - | - |
| T26 | N | N | N | - | - | - |
| T27 | - | - | - | - | - | - |
| T28 | N | N | N | - | - | - |
| T29 | - | - | - | - | - | - |
| T30 | P | N | P | He | Lu, SP, Li | NA |
P = positive; N = negative; OS = oral swab; Lu = lung; To = tonsil; To = tongue; Ki = Kidney; He = heart; Li = liver; Sp = spleen; ML = mandibular lymph node; Il = ileum; Bl = bladder; NA = neutralizing antibodies. (-) represents the times when there was no samples collection.