Fig 3. Effects of single binding site changes on absolute levels, timing or variability of stoichiometry.
(a) A scheme of the promoters of Rec8, Dmc1 and Mei5. Gray bars denote the predicted +1 and -1 nucleosome locations. (b,c) Relative abundance over time in single cells of the pRec8-YFP, pRec8-CFP (b) and pRec8-YFP, pRec8(-MBFbs)-CFP (c) promoter fusion strains. (d, e) Relative stoichiometry over time in single cells of the pMei5-YFP, pMei5-CFP (d) or pMei5-YFP, pMei5(-UME6bs)-CFP (e) strains. (f) A summary of the motif change experiments (see also S3 Fig). For each comparative experiment the mean and standard deviation of ΔT50 and of log(YFP/CFP) in the terminal time point are shown. Removal of MBF binding site decreases pRec8 activity uniformly, with no significant effect on activation timing. Removal (or addition) of a second Ume6 binding site from pMei5 (or pDmc1) also affects mostly the absolute level.