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. 2015 May 22;11(5):e1004933. doi: 10.1371/journal.ppat.1004933

Fig 2. The role of the phosphorylation state of VxrB in colonization.

Fig 2

(A) Amino acid sequence alignment of the REC domains of the RR proteins belonging to the OmpR family using ClustalW. Numbers above the sequence correspond to the amino acid number of each protein. VC numbers indicate the RR encoded in V. cholerae genome. OmpR from E. coli was used as a reference to align the aspartate residue that has been shown to be important for phosphorylation (D55A) [48]. Highlighted region in gray indicate the aspartate residue that is predicted to be the site of phosphorylation. B) In vivo competition assay of a strain harboring the mutated version of vxrB on the chromosome. The vxrB gene was mutated to convert the aspartate residue predicted to be important for phosphorylation to emulate the inactive (D78A) or active (D78E) state of VxrB. *, p<0.05 by the Wilcoxon Signed Rank Test as compared to the CI of wt.