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. 2015 May 22;10(5):e0127386. doi: 10.1371/journal.pone.0127386

Fig 2. ROS-dependent apoptosis in TRAIL-treated MiaPaCa-2 cells.

Fig 2

(A) MiaPaCa-2 and BxPC-3 cells were cultured with TRAIL (50 ng/mL) with or without NAC (10 mM) for 24 h. After staining with annexin V-FITC/PI, flow cytometric analysis was performed. The numbers represent the proportions of each subset. (B) The percentages of annexin V+ cells are shown. (C) Both cell lines were cultured with TRAIL (50 ng/mL) with or without Tempol (1 mM) for 24 h and analyzed by flow cytometry. (D) The percentages of annexin V+ cells are shown. All data points shown represent the mean of three culture wells. *P<0.05, **P<0.01, N.S., not significant.