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. 2015 May 22;10(5):e0127386. doi: 10.1371/journal.pone.0127386

Fig 3. TRAIL-induced necroptosis in human pancreatic cancer cells under ROS inhibition.

Fig 3

(A) The percentages of annexin V-/PI+ cells were calculated based on the results of Fig 2. (B) Both cell lines were cultured with TRAIL (50 ng/mL) and NAC (10 mM), with or without necrostatin-1 (20 μM) for 24 h. After staining with annexin V-FITC/PI, flow cytometric analysis was performed. The numbers represent the proportions of each subset. (C) The percentages of annexin V-/PI+ cells were calculated. All data points shown represent the mean of three culture wells. *P<0.05, **P<0.01.