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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Med Microbiol Immunol. 2015 Feb 28;204(3):449–460. doi: 10.1007/s00430-015-0395-0

Fig. 4.

Fig. 4

Visualization of vMIA clustering on the OMM by MSIM imaging. HeLa cells were transfected with vectors expressing vMIA-EGFP (green) and mitochondrial marker Mito-BFP (pseudocolored red) using Lipofectamine 2000. Transfected cells were fixed with 4 % paraformaldehyde and imaged using MSIM as described [93]. Zoom of the regions R1 and 2 on the merged image show the clustered distribution of vMIA-EGFP at the OMM. The scale bars represent 15 µm on the merged full-size image and 1 µm on the zoomed images