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. Author manuscript; available in PMC: 2015 May 22.
Published in final edited form as: Thromb Haemost. 2012 Feb 8;107(4):717–725. doi: 10.1160/TH11-11-0795

Figure 4. Binding of HIT antibodies.

Figure 4

(A) Effects of excess ODSH on HIT antibody binding to PF4 antigenic complexes. HIT antibodies (1–5) or control subjects (1–2) were tested in a commercial HIT ELISA in the presence of increasing amounts of ODSH (0.5–10 µg/mL) in diluent buffer supplied by the manufacturer. Each data point corresponds to mean ± SD of duplicate wells and is representative of three independent experiments (B) Cross-reactivity of HIT antibodies with PF4/ODSH complexes. HIT antibodies (6–10), control subject and KKO, a murine HIT-like monoclonal antibody, were incubated with wells coated with human PF4 and increasing amounts of ODSH (0.4–3.2 µg/mL) and binding was compared to wells coated with huPF4/UFH. Each data point corresponds to mean ± SD of duplicate wells and is representative of two independent experiments.