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. Author manuscript; available in PMC: 2015 May 23.
Published in final edited form as: Methods Mol Biol. 2013;1008:357–388. doi: 10.1007/978-1-62703-398-5_13

Fig. 12.

Fig. 12

Typical protein immobilization sensogram is shown as a plot of Response Unit (RU) against time. The following protein amine coupling procedure was used: 1 min equilibration followed by 7 min EDC/NHS activation; 2 min equilibration followed by 5 min protein injection; 2 min equilibration followed by 5 min ethanolamine capping; 5 min equilibration. A final coupling of 15,000 RU of a protein was achieved. One resonance unit (RU) represents the binding of approximately 1 pg protein/mm2 and thus it is proportional to the mass of protein bound (36)