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. Author manuscript; available in PMC: 2015 May 23.
Published in final edited form as: Chem Res Toxicol. 2013 Nov 14;26(12):1832–1839. doi: 10.1021/tx400212q

Figure 3.

Figure 3

MS detection of 4HNE modification in Δ51-RGS4(WT) and Δ51-RGS4(Δ7). (a) Injection of 10 μL of RGS4, about 0.7 pmol, was analyzed by LC-ESI-IT-TOF. Expected MW for the deconvoluted singly charged molecular weight was 18090.7 and a detected deconvoluted signal was 18090.8. Treatment with 4HNE revealed a deconvoluted mass was 18558.1. The calculated difference in the mass, 467.3, accounts for three adduction sites for 4HNE. (b) Expected MW for the deconvoluted Δ51-RGS4(Δ7). Singly charged molecular weight was 17869, as detected. After 4HNE treatment, the detected deconvoluted mass was 17869, indicating no detectable modification. The data shown are representative of n = 3 experiments.