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. Author manuscript; available in PMC: 2015 May 23.
Published in final edited form as: Chem Res Toxicol. 2013 Nov 14;26(12):1832–1839. doi: 10.1021/tx400212q

Figure 4.

Figure 4

MS detection of 4HNE treated Δ51-RGS4(WT) tryptic digest. The elution gradient of 5–90% ACN allowed for separation of tryptic peptides for analysis. (a) Outlined is the total ion chromatogram as well as the base peak for each fragment. Peaks are identified according to fragment number from Table 1. (b) The elution peak for each of the adducted fragments. (c) Fragment 2, corresponding to C71, was detected containing a 4HNE adduct. The adducted fragment was detected as a triply charged species with an m/z of 767.99. (d) Fragment 6, corresponding to C148, was detected containing a 4HNE adduct. The adducted fragment was detected as a doubly charged species with an m/z of 948.88. (e) Fragment 8, corresponding to C183, was detected containing a 4HNE adduct. The adducted fragment was detected as a doubly charged species with an m/z of 901.37. The data shown are representative of n = 3 experiments.