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. Author manuscript; available in PMC: 2016 Jun 1.

Published in final edited form as: Free Radic Biol Med. 2015 Feb 4;83:101–114. doi: 10.1016/j.freeradbiomed.2015.01.029

Effect of NONO-aspirin prodrugs on E-cadherin expression in MDA-MB-231 cells. The cells were treated with vehicle (black) or 100 μM IPA/NO-aspirin (blue) or DEA/NO-aspirin (green) for 3, 6 or 12 h at 37 °C. Relative A) mRNA or B) protein expression was measured using RT-PCR and Western blot techniques, respectively. E-cadherin mRNA and protein levels were quantified with respect to control (HPRT), which was treated as 1 for mRNA and 100% for protein. In A, data are plotted as mean ± SD (n = 3).

Effect of NONO-aspirin prodrugs on E-cadherin expression in MDA-MB-231 cells. The cells were treated with vehicle (black) or 100 μM IPA/NO-aspirin (blue) or DEA/NO-aspirin (green) for 3, 6 or 12 h at 37 °C. Relative A) mRNA or B) protein expression was measured using RT-PCR and Western blot techniques, respectively. E-cadherin mRNA and protein levels were quantified with respect to control (HPRT), which was treated as 1 for mRNA and 100% for protein. In A, data are plotted as mean ± SD (n = 3).