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. Author manuscript; available in PMC: 2016 Jun 1.

Published in final edited form as: Free Radic Biol Med. 2015 Feb 4;83:101–114. doi: 10.1016/j.freeradbiomed.2015.01.029

Effect of NONO-aspirin prodrugs on caspase-3 activity of A) MDA-MB-231, B) MDA-MB-468 or C) MCF-7 cells. The cells were treated with various concentrations (0, 25, 50, 100 μM) of IPA/NO-aspirin (blue) or DEA/NO-aspirin (green) for 24 h at 37 °C. Caspase-3 activity was determined by recording the fluorescence (λ_ex 485 nm, λ_em 535 nm) of the product formed by cleavage of the substrate N-Ac-DEVD-N′-MC-R11. Data are plotted as mean ± SD (n = 3).

Effect of NONO-aspirin prodrugs on caspase-3 activity of A) MDA-MB-231, B) MDA-MB-468 or C) MCF-7 cells. The cells were treated with various concentrations (0, 25, 50, 100 μM) of IPA/NO-aspirin (blue) or DEA/NO-aspirin (green) for 24 h at 37 °C. Caspase-3 activity was determined by recording the fluorescence (λ_ex 485 nm, λ_em 535 nm) of the product formed by cleavage of the substrate N-Ac-DEVD-N′-MC-R11. Data are plotted as mean ± SD (n = 3).