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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2015 Feb 17;83:139–148. doi: 10.1016/j.freeradbiomed.2015.01.035

Figure 5. Lower NF-κB nuclear translocation in MKK3−/− BMDMs after LPS stimulation.

Figure 5

BMDMs were treated with LPS (0.1 μg/ml) for 45 min and immuno-stained with p-NF-κB antibody. Slides where imaged with confocal microscopy and quantified using CellProfiler software. There was an increase in nuclear p-NF-κB in WT but to a lesser extent in MKK3−/− BMDMs compared to respective controls. * represents significance at p<0.05. Data is representative of 3 experiments done in at least duplicates.