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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2015 Feb 26;83:227–237. doi: 10.1016/j.freeradbiomed.2015.02.018

Figure 5. MnP + AscH− and gemcitabine does not alter systemic levels of 4HNE-modified proteins in vivo.

Figure 5

(A) Representative 4HNE dot blot. Purified bovine serum albumin (BSA) was reacted with excess purified 4-HNE to create a positively labeled protein control seen in the upper panel. Negative control is unreacted purified BSA. Blot for 4-HNE modified proteins in cardiac muscle showed no changes in immunoreactive protein after any of the treatments when compared to controls.

(B) Quantification of dot blots for 4-HNE modified proteins in mouse heart, liver and kidney homogenates demonstrated no difference in immuno-reactive protein after any of the treatments when compared to controls; n = 3.