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PCR-RFLP pattern of Fasciola after digestion with Rsa I restriction enzyme. Lane M: 100bp DNA ladder, Lanes 7 F. gigantic, from cattle, Lane 9: PCR amplicons (upper) of F. gigantic and their restriction fragments (lower) produced by the PCR-RFLP method.from cattle, Lane 11 and 12: F. hepatica from sheep, Lane 14: F. gigantica from cattle before digestion with Rsa I restriction enzyme
