Table 1.
Outline of experimental groups for the nicotine + ethanol sensitization studies
| Baseline | Acquisition | Ethanol Challenge | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Treatment Groups Day 3–12 (Day 13) | Day 1–2 | Day 3 | Day 4–5 | Day 6 | Day 7–8 | Day 9 | Day 10–11 | Day 12 | Day 13 |
| 1 g/kg ethanol groups | |||||||||
| SAL (E1) | SAL | SAL | SAL | SAL | SAL | SAL | SAL | SAL | (E1) |
| N1 (E1) | SAL | N1 | N1 | N1 | N1 | N1 | N1 | N1 | (E1) |
| N2 (E1) | SAL | N2 | N2 | N2 | N2 | N2 | N2 | N2 | (E1) |
| E1 (E1) | SAL | E1 | E1 | E1 | E1 | E1 | E1 | E1 | (E1) |
| N1+E1 (E1) | SAL | N1+E1 | N1+E1 | N1+E1 | N1+E1 | N1+E1 | N1+E1 | N1+E1 | (E1) |
| N2+E1 (E1) | SAL | N2+E1 | N2+E1 | N2+E1 | N2+E1 | N2+E1 | N2+E1 | N2+E1 | (E1) |
| 2 g/kg ethanol groups | |||||||||
| SAL (E2) | SAL | SAL | SAL | SAL | SAL | SAL | SAL | SAL | (E2) |
| N1 (E2) | SAL | N1 | N1 | N1 | N1 | N1 | N1 | N1 | (E2) |
| N2 (E2) | SAL | N2 | N2 | N2 | N2 | N2 | N2 | N2 | (E2) |
| E2 (E2) | SAL | E2 | E2 | E2 | E2 | E2 | E2 | E2 | (E2) |
| N1+E2 (E2) | SAL | N1+E2 | N1+E2 | N1+E2 | N1+E2 | N1+E2 | N1+E2 | N1+E2 | (E2) |
| N2+E2 (E2) | SAL | N2+E2 | N2+E2 | N2+E2 | N2+E2 | N2+E2 | N2+E2 | N2+E2 | (E2) |
| Activity test | Yes | Yes | No | Yes | No | Yes | No | Yes | Yes & Blood |
“E1” or “E2” = 1 or 2 g/kg ethanol. “SAL”= 0.9% Saline. “N1” or “N2” = 1 or 2 mg/kg of nicotine tartrate. Yes = activity test occurs. No = no activity test.