FIG 7 .

Contributions of the different subunits of the Arp2/3 complex to actin comet tail formation by Listeria. (A) Examples of images from validation experiments for actin and Listeria upon inactivation of each subunit of the Arp2/3 complex. Bar, 5 µm (B) Percentage of Listeria cells displaying long actin tails, short tails, full actin clouds, partial clouds, or no actin association upon inactivation of Arp2, Arp3, p41 (both subunits simultaneously), p34, p21, p20, and p16. Bars represent the means from 4 experiments. (C) HeLa cells infected with Listeria for 6 h and stained for actin (red) and for the Arp2/3 complex subunit p20 (left panel, green) or p16 (right panel, green). Bars, 5 µm; insert bar left, 2 µm; insert bar right, 1 µm.