Figure 5.

ROS staining ((a) on radicle tips; (b) on maturation zone; bar: 200 μm), Evans blue staining (c), and hematoxylin staining (d) in wheat radicles. Initially, wheat grains were geminated in water for 36 h. Then four treatment groups were done as follows, control, 60 h in H₂O; Pre-S, pretreatment with 1 mM SO₂ donor for 12 h, and then exposed to H₂O for 48 h; Al, 12 h in H₂O prior to exposure to 400 μM AlCl₃ for 48 h; Pre-S + Al, 12 h in 1 mM SO₂ donor pretreatment followed by 400 μM AlCl₃ for 48 h.