Effect of PA-X on viral replication in vitro. A549, Vero, MDCK, DF-1, CEF, and DEF cells were inoculated at an MOI of 0.01. Virus titers were determined in MDCK cells at the indicated time points. Data are means ± SD for three independent infections. Asterisks indicate significant differences between CK-PAX3 and r-CK10 (*, P < 0.05; **, P < 0.01).