FIG 5.
Downregulation of PA-X expression increases the host global transcriptional response. (A) Number of genes SDE (with ≥2-fold change at a P value of <0.05) during infection with 103.0 EID50 of r-CK10 or CK-PAX3 relative to mock infection at 12 and 24 h p.i. (B) Venn diagram showing the distribution of SDE genes. The Venn diagrams were generated using Venny (www.pangloss.com/seidel/Protocols/venn.cgi). (C and D) Gene Ontology analysis of the SDE genes using IPA at 24 h p.i. (C) Top biofunctions of the SDE genes. (D) Top canonical pathways of the SDE genes. (E and F) Expression patterns of inflammatory response- and cell death-related genes in chicken lungs. Heat maps show the hierarchical clustering of the expression profiles of genes related to the inflammatory response (E) and cell death (F) during infection with r-CK10 or CK-PAX3 at 24 h p.i. Clustering was performed by the hierarchical unweighted-pair group method using average linkages with a Euclidean distance measure. The expression of the genes for which data are displayed was changed ≥2-fold (P < 0.05) from that in mock-infected controls.