FIG 6.

Loss of PA-X expression augments the innate immune response in chicken lungs. (A and B) Hierarchical clustering of expression profiles of innate immune response-related genes during infection with r-CK10 or CK-PAX3 at 12 or 24 h p.i. (A) Genes involved in the activation or trafficking of immune cells (including NK cells, macrophages, dendritic cells [DC], antigen-presenting cells [APC], and T lymphocytes) and cytokine responses. (B) Interferon (IFN) and IFN-related genes. Clustering was performed by the hierarchical unweighted-pair group method using average linkages with a Euclidean distance measure. The expression of the genes for which data are displayed was changed ≥2-fold (P < 0.05) from that in mock-infected controls at least at one time point of infection. (C) Modeling of the IFN response pathway in the lungs of chickens infected with CK-PAX3 at 24 h p.i. (red, upregulated; green, downregulated).