TABLE 1.
Primers for amplification of cytokine genes
| Host and primer name | Sequence (5′–3′) | GenBank accession no. of target |
|---|---|---|
| Duck | ||
| GAPDHF | ATGTTCGTGATGGGTGTGAA | AY436595 |
| GAPDHR | CTGTCTTCGTGTGTGGCTGT | |
| MXF | TCACACGAAGGCCTATTTTACTGG | NM_204609 |
| MXR | GTCGCCGAAGTCATGAAGGA | |
| IL-8F | AGGACAACAGAGAGGTGTGCTTG | NM_205498 |
| IL-8R | GCCTTTACGATCCGCTGTACC | |
| IL-1βF | GAGATTTTCGAACCCGTCACC | DQ393268 |
| IL-1βR | AGGACTGGGAGCGGGTGTA | |
| IL-18F | AGGTGAAATCTGGCAGTGGAAT | NM_204608.1 |
| IL-18R | ACCTGGACGCTGAATGCAA | |
| IFN-α-F | TTGCTCCTTCCCGGACA | EF053034 |
| IFN-α-R | GCTGAGGGTGTCGAAGAGGT | |
| IFN-β-F | CCTCAACCAGATCCAGCATT | AY831397 |
| IFN-β-R | GGATGAGGCTGTGAGAGGAG | |
| Chicken | ||
| β-actinF | ATTGTCCACCGCAAATGCTTC | NM_205518.1 |
| β-actinR | AAATAAAGCCATGCCAATCTCGTC | |
| IFN-βF | TGCACAGCATCCTACTGCTCTTG | NM_001024836.1 |
| IFN-βR | GTTGGCATCCTGGTGACGAA | |
| IFN-rF | CTCCCGATGAACGACTTGAG | NM_205149.1 |
| IFN-rR | CTGAGACTGGCTCCTTTTCC | |
| MX-1F | ATCCATGGTCCAACTTCAGC | NM_204609.1 |
| MX-1R | GCCTCTTGGACACTTTCTGC | |
| IL-6F | TTCGCCTTTCAGACCTACCT | EU170468 |
| IL-6R | TGGTGATTTTCTCTATCCAGTCC | |
| IL-18F | AGGTGAAATCTGGCAGTGGAAT | NM_204608.1 |
| IL-18R | ACCTGGACGCTGAATGCAA | |
| CCL-19F | CCAGGAAGGTCCCAAATCAA | XM_424980.4 |
| CCL-19R | ACCACAGCAGGACATAGAAGCA |