TABLE 2.
Total yield of NAI-susceptible and -resistant recombinant influenza B viruses following coinfection of NHBE cells in the absence or presence of NAIs
| Virus in mixturea | Mean AUC (degree of restriction after treatment with NAI)b |
||||
|---|---|---|---|---|---|
| No Drug | Oseltamivir |
Zanamivir |
|||
| 1 μM | 10 μM | 1 μM | 10 μM | ||
| rg-E119A | 303.2 | 287.6 (15.6) | 237.2 (66.0)** | 280.4 (22.8) | 233.2 (70.0)** |
| rg-D198E | 299.2 | 278.8 (20.4) | 249.8 (49.4) | 169.6 (129.6)† | 130.4 (168.8)† |
| rg-I222T | 304.8 | 270.8 (34.0) | 231.2 (73.6) | 118 (186.8)** | 0 (304.8)† |
| rg-H274Y | 282.0 | 267.2 (14.8) | 272.8 (9.2) | 169.6 (112.4)† | 0 (282.0)† |
| rg-N294S | 276.8 | 253.6 (23.2) | 165.6 (111.2)† | 209.2 (67.6)* | 172.8 (104.0)† |
| rg-R371K | 272.4 | 278.8 (−6.4) | 290.8 (−18.4) | 147.2 (125.2)* | 13.2 (259.2)† |
a
Mixture of rg-WT and a recombinant influenza B virus carrying the indicated single substitution in NA.
b
The mean AUC indicates the total virus load at 24, 48, and 72 h p.i. The degree of restriction indicates the difference in the mean AUC between the control group (no drug) and the group treated with NAIs. *, P < 0.05; **, P < 0.01; †, P < 0.001 compared to the group not treated with drug by one-way ANOVA followed by Dunnett's multiple-comparison test.