FIG 2.

CP inhibits translation in a dose-dependent manner. The dose of CP expressed in Nicotiana benthamiana plants is proportional to the level of inhibition of translation. (A) Plants were infiltrated with increasing amounts (OD₆₀₀s of 0.05, 0.1, and 0.3) of Agrobacterium carrying a T-DNA to express CP. Twenty-four hours later, the same plants were infiltrated with PVA^wt. GUS was used as a control, as it does not interfere with viral gene expression. GUS expression was initiated by Agrobacterium infiltration (OD₆₀₀ = 0.3). Student's t test was carried out to study the significance of the difference between GUS and CP^wt expression with PVA^wt. **, P < 0.01. (B) CP expression was initiated similarly to that in panel A, with various amounts of Agrobacterium. Total protein from infiltrated leaves was extracted and analyzed via Western blotting. Anti-CP was used for immunodetection of CP. Mock-infected Nicotiana benthamiana plants were used as a negative control. A protein band corresponding to RubisCO on a Ponceau S-stained membrane was used as a loading control.