FIG 7.

Transmission electron microscopy (TEM) and immunocapture RT-PCR. Virion formation was assessed in Nicotiana benthamiana plants coexpressing PVA^wt with GUS (control plants) or CP^wt (experimental plants) at 10 dpi. (A) Virions contained in both control and experimental plants were captured on carbon-coated grids, followed by negative staining with 3% uranyl acetate for visualization on a JEOL 1400 TEM. Virions similar in shape and size were detected in both plants. (B) Immunocapture RT-PCR was used to investigate the RNA content of the virus particles. Control and experimental plants both contained the full-length RNA molecule.