FIG 4.

Effects of internal deletion on ICP0 functional domains located in its N and C termini. (A) ICP0 internal deletion does not affect its ability to interact with USP7. HEp2 cells were infected with recombinant viruses RHG101, RHG104, RHG110, and RHG127 at 5 PFU/cell for 14 h. Infected-cell lysates were then subjected to a coimmunoprecipitation (IP) assay with polyclonal anti-USP7 antibody. The immunoprecipitates were electrophoretically separated on SDS-PAGE gels and probed with the indicated antibodies by Western blotting (WB). (B) ICP0 internal deletion diminishes its E3 function in PML degradation. HEL and HEp2 cells were mock infected or infected with recombinant viruses RHG101, RHG110, and RHG127 at 10 PFU/cell. At 8 h postinfection, total cell lysates were probed with the indicated antibodies by Western blotting.