FIG 3.

Fold regulation of selected genes related to human inflammatory cytokines and receptors following influenza virus infection of human lung epithelial cells. Calu-3 cells were infected with influenza viruses at an MOI of 1 in triplicate. Total RNA was isolated from cells at 24 h p.i. and examined by real-time RT-PCR array analysis. Selected genes which exhibited a significant (P < 0.05), >3-fold change over or under that in mock-infected cells are shown. (A) Selected genes (n = 17) for which infection with VN/1203 virus elicited significantly higher transcript levels than did Anhui/1 or Texas/50 virus infection. (B) Selected genes (n = 17) for which infection with Anhui/1 virus elicited transcript levels comparable to those elicited by VN/1203 virus infection.