FIG 9.

Replication kinetics study of both human and avian influenza viruses in differentiated primary human bronchial and tracheal epithelial cells. HTE cells were infected apically with virus at an MOI of 0.01 and cultured at either 37°C or 33°C for the duration of the time course. At each time point, 200 μl of medium was added to the apical surface for 20 min and collected for viral titer determination. Values represent the means of three independent experiments plus standard deviations. (A) Replication of viruses at 37°C; (B) replication of Brisbane/59 virus at 37°C and 33°C; (C) replication of Anhui/01 virus at 37°C and 33°C; (D) replication of VN/1203 virus at 37°C and 33°C. *, virus replicated to a significantly higher titer at 37°C than at 33°C (P < 0.05) at 24 h and 48 h p.i. according to the area under the curve (AUC).