TABLE 3.
Steady-state kinetic parameters for recombinant wild-type and mutant UL30 and UL54 DNA polymerases and inhibition of enzyme activity by foscarneta
| Mutation | Steady-state kinetic parameters |
Inhibition by foscarnet |
||||
|---|---|---|---|---|---|---|
| Vmax(app) (fmol/min) | Km(app) (μM) | Vmax(app)/Km(app) | Fold changeb | Ki(app) (μM) | Fold changeb | |
| UL30 | ||||||
| WT | 61 | 0.48 | 127 | 1 | 0.04 | 1 |
| W781V | 9.2 | 0.77 | 12 | 0.09 | 1.8 | 45 |
| UL54 | ||||||
| WT | 8.6 | 0.55 | 16 | 1 | 0.55 | 1 |
| W780V | 3.7 | 0.99 | 3.7 | 0.23 | 2.7 | 4.9 |
a
Results are representative of those from two independent experiments. WT, wild type.
b
Fold change in Vmax(app)/Km(app) values or Ki(app) values compared to the values for the wild-type DNA polymerase.