FIG 5.

NSs captures STAT2 and STAT1 into viral inclusion bodies (IBs). HepG2 cells were transfected with the plasmid expressing C-terminally HA-tagged NSs (A) or infected with SFTSV at an MOI of 1 (B). Twenty-four h later, cells were fixed to visualize the subcellular localization of NSs (green) and endogenous STAT2 or STAT1 (red) by IFA. NSs was stained with the anti-HA antibody for transfected cells (A) or the anti-NSs antiserum for SFTSV-infected cells (B), respectively, while STATs were visualized with the corresponding anti-STAT antibodies. Nuclei stained with Hoechst are shown in blue.