FIG 7.

NSs inhibits STAT2 phosphorylation. (A) HEK293 cells transfected with the NSs expression plasmid or the vector were treated with IFN-α (1,000 U/ml) or left untreated for 30 min and were harvested to evaluate the levels of phosphorylated or total STATs by WB using the antibodies against the indicated proteins. (B) HEK293 cells were infected with SFTSV at an MOI of 5. At 24 h p.i., cells were treated with IFN-α (1,000 U/ml) or left untreated for 30 min and harvested for WB as described for panel A.